Difference between revisions of "Part:BBa R0011"

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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_R0011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_R0011 SequenceAndFeatures</partinfo>
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Intrinsic Noise Value: 0.0040 (compare with R0010: 0.0707; R0051: 0.0869). See [http://2015.igem.org/Team:William_and_Mary William_and_Mary iGEM 2015]
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 01:38, 19 September 2015

Promoter (lacI regulated, lambda pL hybrid)

Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be:

  • repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]).
  • induced by [http://openwetware.org/wiki/IPTG IPTG] in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range


Usage and Biology

Strong promoter. [jb, 5/24/04]

R0011 will be on in strains without lacI, off in strains that are lacIq (ie. Part:BBa_V1003) and medium in strains that are lacI

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]