Difference between revisions of "Part:BBa K1583015"
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<p>A linker with the protein sequence GSGGSG was placed in between the CsgA (<a href="https://parts.igem.org/Part:BBa_K1583000"target="_blank">BBa_K1583000</a>) and the affinity tags, His and Hydroxyapatite, for increased performance reasons. The design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres" | <p>A linker with the protein sequence GSGGSG was placed in between the CsgA (<a href="https://parts.igem.org/Part:BBa_K1583000"target="_blank">BBa_K1583000</a>) and the affinity tags, His and Hydroxyapatite, for increased performance reasons. The design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres" | ||
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<span class='h3bb'><h3>Sequence and Features</h3></span> | <span class='h3bb'><h3>Sequence and Features</h3></span> |
Revision as of 01:04, 19 September 2015
Linker in fusion protein
A linker with the protein sequence GSGGSG was placed in between the CsgA (BBa_K1583000) and the affinity tags, His and Hydroxyapatite, for increased performance reasons. The design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres"
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.