Difference between revisions of "Part:BBa K1602042"

 
 
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<partinfo>BBa_K1602042 short</partinfo>
 
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                This part is capable to generate the enhanced yellow fluorescent protein <b>(eYFP) </b> codon optimized for usage in <i>E. coli</i> (<a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>) and under control of a T7-promotor. To fuse the protein to an <i>in vitro</i> scaffold (<a href="https://parts.igem.org/Part:BBa_K1602027">BBa_K1602027</a>) a linker-ligand construct was added. This ligand binds to the PDZ domain of the scaffold.
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Latest revision as of 02:57, 19 September 2015

Inducible generator of eYFP with C-terminal PDZ ligand

This part is capable to generate the enhanced yellow fluorescent protein (eYFP) codon optimized for usage in E. coli (BBa_B0034) and under control of a T7-promotor. To fuse the protein to an in vitro scaffold (BBa_K1602027) a linker-ligand construct was added. This ligand binds to the PDZ domain of the scaffold.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]