Difference between revisions of "Part:BBa K1758101:Design"

 
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===Design Notes===
 
===Design Notes===
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<a href="http://www.ncbi.nlm.nih.gov/pubmed/23654270">Lentini et al. 2013</a> showed that the scar which is created by standard biobrick assembly is disadvantageous for <i>in vitro</i> translation when occuring between RBS and start codon. Therefore we changed the sequence between RBS and start codon for other parts.  
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[http://www.ncbi.nlm.nih.gov/pubmed/23654270 Lentini et al. 2013] showed that the scar which is created by standard biobrick assembly is disadvantageous for ''in vitro'' translation when occuring between RBS and start codon. Therefore we changed the sequence between RBS and start codon for other parts.
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===Source===
 
===Source===

Revision as of 13:39, 17 August 2015


Translation enhancing 5-UTR + sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 59


Design Notes

[http://www.ncbi.nlm.nih.gov/pubmed/23654270 Lentini et al. 2013] showed that the scar which is created by standard biobrick assembly is disadvantageous for in vitro translation when occuring between RBS and start codon. Therefore we changed the sequence between RBS and start codon for other parts.

Source

This part was created by shortening <a href="https://parts.igem.org/Part:BBa_I746909">BBa_I746909</a> and simultaneously adding 5'-UTR via Gibson primers.

Amplification with Gibson-primers:


References