Difference between revisions of "Part:BBa J45992:Design"

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===References===
 
===References===
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<biblio>
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#Yim pmid=8282684
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#Schellhorn pmid=9829938
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#Wise pmid=8631665
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#Funabashi pmid=11879714
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#Makinoshima pmid=11985708
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</biblio>
  
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Nucleotide&list_uids=453110&dopt=GenBank
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http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8282684
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http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=9829938
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http://www.blackwell-synergy.com/doi/abs/10.1046/j.1365-2958.2002.02746.x
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http://www.pubmedcentral.org/articlerender.fcgi?artid=178012
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http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3C-456T3CY-3&_coverDate=04%2F25%2F2002&_alid=423325879&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=4943&_sort=d&view=c&_acct=C000022659&_version=1&_urlVersion=0&_userid=501045&md5=7d6f4bac2242f1baa6b2b15260904531
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Neidhart, Frederick C. ''Escherichia coli and Salmonella''. Washington: ASM Press, 1996.
 
Neidhart, Frederick C. ''Escherichia coli and Salmonella''. Washington: ASM Press, 1996.

Revision as of 23:54, 2 February 2008


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Design Notes

Primers used to PCR this part out of the E.coli genome:

Forward Primer

  • 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3'
  • Total Length: 47 bp
  • Annealing Length: 19 bp
  • GC Content: 52.6%
  • Melting Temperature: 53.4 degrees C
  • hairpin deltaG: -3.99 kcal/mol
  • self dimer deltaG: -98.3 kcal/mol

Reverse Primer

  • 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3'
  • Total Length: 54 bp
  • Annealing Length: 25 bp
  • GC Content: 20.0%
  • Melting Temperature: 46.4 degrees C
  • hairpin deltaG: -2.86 kcal/mol
  • self dimer deltaG: -99.44 kcal/mol
  • heterodimer deltaG with Forward Primer: -99.44 kcal/mol

Source

This part was PCRed out of E. coli's genome with the primers listed in the design notes section.

References

<biblio>

  1. Yim pmid=8282684
  2. Schellhorn pmid=9829938
  3. Wise pmid=8631665
  4. Funabashi pmid=11879714
  5. Makinoshima pmid=11985708

</biblio>

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.