Difference between revisions of "Part:BBa J45992:Experience"
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===Applications of BBa_J45992===
 
===Applications of BBa_J45992===
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A stationary phase-dependent PoPS source.
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J45992 was characterized by measuring flourescence intensity of the in a plate reader.  The characterization device was <bbpart>BBa_J45995</bbpart>.  In figure A, the absorbance, which is proportional to cell density, is plotted against fluorescence, or roughly the number of GFP molecules present in the culture.  It is seen in the graph that J45995 (J45992.E0840) exhibits a much higher level of fluorescence during stationary phase (Absorbance>0.5, see figure B) than during exponential phase (yellow line).  Additionally, J45992 was attached to an inverter (<bbpart>BBa_Q04401</bbpart>) and the signal was effectively turned off at stationary phase (green line).  
 
J45992 was characterized by measuring flourescence intensity of the in a plate reader.  The characterization device was <bbpart>BBa_J45995</bbpart>.  In figure A, the absorbance, which is proportional to cell density, is plotted against fluorescence, or roughly the number of GFP molecules present in the culture.  It is seen in the graph that J45995 (J45992.E0840) exhibits a much higher level of fluorescence during stationary phase (Absorbance>0.5, see figure B) than during exponential phase (yellow line).  Additionally, J45992 was attached to an inverter (<bbpart>BBa_Q04401</bbpart>) and the signal was effectively turned off at stationary phase (green line).  
  
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<partinfo>BBa_J45992 AddReview 5</partinfo><br>
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<I>[[User:Rshetty|Reshma Shetty]]</I>
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<partinfo>BBa_J45992</partinfo>, when used to regulate transcription of GFP in <partinfo>BBa_J45995</partinfo> or banana odor enzyme generator in <partinfo>BBa_J45250</partinfo>, demonstrated the expected behavior.  <partinfo>BBa_J45992</partinfo> produced a low transcriptional signal in exponential phase and a high transcriptional signal in stationary phase.
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Revision as of 23:52, 7 March 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J45992

A stationary phase-dependent PoPS source.

J45992 was characterized by measuring flourescence intensity of the in a plate reader. The characterization device was BBa_J45995. In figure A, the absorbance, which is proportional to cell density, is plotted against fluorescence, or roughly the number of GFP molecules present in the culture. It is seen in the graph that J45995 (J45992.E0840) exhibits a much higher level of fluorescence during stationary phase (Absorbance>0.5, see figure B) than during exponential phase (yellow line). Additionally, J45992 was attached to an inverter (BBa_Q04401) and the signal was effectively turned off at stationary phase (green line).

Figure A - J45992 chracterization (yellow), with signal inverted (green), with control constitutive promoter (grey), and with no fluorescent protein coding region (purple)
Figure B - Graph shows that cell reach stationary phase at absorbance ~0.5


User Reviews

UNIQ0a1900f9f00cee92-partinfo-00000002-QINU

•••••

Reshma Shetty

BBa_J45992, when used to regulate transcription of GFP in BBa_J45995 or banana odor enzyme generator in BBa_J45250, demonstrated the expected behavior. BBa_J45992 produced a low transcriptional signal in exponential phase and a high transcriptional signal in stationary phase.

UNIQ0a1900f9f00cee92-partinfo-00000008-QINU