Difference between revisions of "Part:BBa K1330002:Design"
 
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===Design Notes===
 
===Design Notes===
  
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This part was constructed by cutting the promoter out of [Part:BBa_J23105|BBa_J23105]] with EcoRI and SpeI, and ligating it with cut (also EcoRI and SpeI) pSB1C3 backbone.
+
This part was constructed by cutting the promoter out of [[Part:BBa_J23105|BBa_J23105]] with EcoRI and SpeI, and ligating it with cut (also EcoRI and SpeI) pSB1C3 backbone.
  
  

Latest revision as of 11:59, 22 October 2014

Constitutive promoter (J23105)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was constructed by cutting the promoter out of BBa_J23105 with EcoRI and SpeI, and ligating it with cut (also EcoRI and SpeI) pSB1C3 backbone.


Source

2014 iGEM Distribution Kit

References