Difference between revisions of "Part:BBa K1321120"

Latest revision as of 03:37, 2 November 2014

SmtA metallothionein fused to linker-dCBD

Metallothionein (metal binding peptide) SmtA fused N-terminally to dCBD (a cellulose-binding domain), which contains an endogenous N-terminal linker sequence.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below:

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

For reference the cellulose binding domain binding capability to bacterial cellulose was measured relative to other cellulose binding domains when fused to sfGFP, the data for which can be seen here (here) - K1321348.

Sequence and Features