Difference between revisions of "Part:BBa K1319016"
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A TEV protease is available codon optimised for ''E. coli'' with the part [https://parts.igem.org/Part:BBa_K1319004 K1319004]. | A TEV protease is available codon optimised for ''E. coli'' with the part [https://parts.igem.org/Part:BBa_K1319004 K1319004]. | ||
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| + | ==Contribution: HKUST 2022== | ||
| + | Team HKUST 2022 uses the TEV protease cleavage site (ENLYFQ cut site) for their experiment on the characterization of their degradation system using TEV. By changing the position of the cleavage site and the LVA tag, HKUST Team 2022 achieve 2 purposes that is to increase output of a certain signal in a shorter period of time and reduce color mixing issue. For more information on how HKUST 2022 Team uses this cleavage site refer to the composite part [https://parts.igem.org/Part:BBa_K4225012 BBa_K4225012] and [https://parts.igem.org/Part:BBa_K4225019 BBa_K4225019] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 11:18, 10 October 2022
TEV protease cleavage site
This sequence codes for a TEV protease cleavage site.
ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.
Usage and Biology
A TEV protease is available codon optimised for E. coli with the part K1319004.
Contribution: HKUST 2022
Team HKUST 2022 uses the TEV protease cleavage site (ENLYFQ cut site) for their experiment on the characterization of their degradation system using TEV. By changing the position of the cleavage site and the LVA tag, HKUST Team 2022 achieve 2 purposes that is to increase output of a certain signal in a shorter period of time and reduce color mixing issue. For more information on how HKUST 2022 Team uses this cleavage site refer to the composite part BBa_K4225012 and BBa_K4225019
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]