Difference between revisions of "Part:pSB1AK8:Design"
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Eukaryotic terminator vector was made by restriction of BioBrick vector pSB1AK3 and ligation of eukaryotic terminator in this vector. We had some problems with ligation of vector itself (without the terminator). | Eukaryotic terminator vector was made by restriction of BioBrick vector pSB1AK3 and ligation of eukaryotic terminator in this vector. We had some problems with ligation of vector itself (without the terminator). | ||
This vector was very important for us, because of its terminator. We cloned all our composite parts in this vector, so all composite parts end with terminator. | This vector was very important for us, because of its terminator. We cloned all our composite parts in this vector, so all composite parts end with terminator. | ||
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| + | *'''[[User:Rshetty|RS]] 23:11, 3 September 2008 (UTC)''': The default insert for this vector is [[Part:BBa_P1010]]. The physical location for this vector needs to be remapped to [[Part:BBa_P1010]]. BBa_J52017 is the vector that contains [[Part:BBa_P1010]]. | ||
===Source=== | ===Source=== | ||
Revision as of 23:11, 3 September 2008
No part name specified with partinfo tag.
No part name specified with partinfo tag.
Design Notes
Eukaryotic terminator vector was made by restriction of BioBrick vector pSB1AK3 and ligation of eukaryotic terminator in this vector. We had some problems with ligation of vector itself (without the terminator). This vector was very important for us, because of its terminator. We cloned all our composite parts in this vector, so all composite parts end with terminator.
- RS 23:11, 3 September 2008 (UTC): The default insert for this vector is Part:BBa_P1010. The physical location for this vector needs to be remapped to Part:BBa_P1010. BBa_J52017 is the vector that contains Part:BBa_P1010.
Source
The source for eukaryotic terminator is pcDNA3 by Invitrogen.