Difference between revisions of "Part:BBa K1319014"
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<partinfo>BBa_K1319014 short</partinfo> | <partinfo>BBa_K1319014 short</partinfo> | ||
| − | This part expresses a [https://parts.igem.org/Part:BBa_E0040 E0040].[https://parts.igem.org/Part:BBa_K1319002 K1319002] fusion protein ( | + | This part expresses a [https://parts.igem.org/Part:BBa_E0040 E0040].[https://parts.igem.org/Part:BBa_K1319002 K1319002] fusion protein (GFP-REACh1) behind a [https://parts.igem.org/Part:BBa_J23101 J23101] promoter. |
[https://parts.igem.org/Part:BBa_K1319002 K1319002] is a '''dark quencher''' that eliminates the fluorescence of the GFP-domain by Förster Resonance Energy Transfer (FRET), but does not exhibit strong fluorescence itself. | [https://parts.igem.org/Part:BBa_K1319002 K1319002] is a '''dark quencher''' that eliminates the fluorescence of the GFP-domain by Förster Resonance Energy Transfer (FRET), but does not exhibit strong fluorescence itself. | ||
Latest revision as of 02:22, 18 October 2014
Constitutive expression of GFP-REACh2 fusion protein
This part expresses a E0040.K1319002 fusion protein (GFP-REACh1) behind a J23101 promoter. K1319002 is a dark quencher that eliminates the fluorescence of the GFP-domain by Förster Resonance Energy Transfer (FRET), but does not exhibit strong fluorescence itself.
Usage and Biology
The two domains can be separated from each other via a TEV protease cleavage site in the linker.
More information about the characterization of this part can be found on the part page of K1319002.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1521
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 706