Difference between revisions of "Part:BBa K1541009"
| Line 2: | Line 2: | ||
<partinfo>BBa_K1541009 short</partinfo> | <partinfo>BBa_K1541009 short</partinfo> | ||
| − | this riboregulated promoter construct contains the quorum sensing promoter [https://parts.igem.org/Part: | + | this riboregulated promoter construct contains the quorum sensing promoter [https://parts.igem.org/Part:BBa_I14017 BBa_I14017] which can be activated in presence of [https://parts.igem.org/Part:BBa_C0171:Experience RhlR (BBa_C0171)] and C4-HSL, the product of the enzyme [https://parts.igem.org/Part:BBa_C0170 RhlI (BBa_C0170)], succeeded by the gene for sfGFP. However, the promoter [https://parts.igem.org/Part:BBa_I14017 BBa_I14017] by itself shows some leakiness. Together with the ribogegulator 12<sup>[[Team:ETH_Zurich/project/references#refCallura|[18]]]</sup> the leakiness could be reduced. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 19:43, 16 October 2014
sfGFP under promoter P(Rhl) with riboregulator RR12
this riboregulated promoter construct contains the quorum sensing promoter BBa_I14017 which can be activated in presence of RhlR (BBa_C0171) and C4-HSL, the product of the enzyme RhlI (BBa_C0170), succeeded by the gene for sfGFP. However, the promoter BBa_I14017 by itself shows some leakiness. Together with the ribogegulator 12[18] the leakiness could be reduced.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 765
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]