Difference between revisions of "Part:BBa K1465228:Design"

(→‎Design Notes)
(→‎Design Notes)
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  '''rv-SBPase-BBa_B0034-pSB1C3 (65 bp)'''<br>
 
  '''rv-SBPase-BBa_B0034-pSB1C3 (65 bp)'''<br>
 
  5'-TTTTCGCTTTTCAATTCCCTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCCAGAAATC-3'<br>
 
  5'-TTTTCGCTTTTCAATTCCCTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCCAGAAATC-3'<br>
 +
 +
*Amplification of the front part of the Sedoheptulose-1,7-Bisphosphatase (''glpX'') for the removal of the illegal recostriction site. This part contains the RBS <bbpart>BBa_B0034</bbpart> and the front part of the Sedoheptulose-1,7-Bisphosphatase from ''Bacillus metanolicus''. For the amplification the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_pSB1C3_csoS4A csoS4A_fwd] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_csoS4A_PstI csoS4A_PstI-rev] were used:<br>
  
 
===Source===
 
===Source===

Revision as of 23:14, 8 October 2014


Sedoheptulose 1,7-bisphosphatase (glpX) from Bacillus methanolicus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Amplification of the pSB1C3-Backbone (BBa_J04450) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw-pSB1C3-BBa_B0034-SBPase fw-pSB1C3-BBa_B0034-SBPase] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv-SBPase-BBa_B0034-pSB1C3 rv-SBPase-BBa_B0034-pSB1C3]:
fw-pSB1C3-BBa_B0034-SBPase (58 bp)

5'-GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGAGGGAATTGAAAAGCGAAAAG-3'

rv-SBPase-BBa_B0034-pSB1C3 (65 bp)

5'-TTTTCGCTTTTCAATTCCCTCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCCAGAAATC-3'
  • Amplification of the front part of the Sedoheptulose-1,7-Bisphosphatase (glpX) for the removal of the illegal recostriction site. This part contains the RBS BBa_B0034 and the front part of the Sedoheptulose-1,7-Bisphosphatase from Bacillus metanolicus. For the amplification the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#fw_pSB1C3_csoS4A csoS4A_fwd] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#rv_csoS4A_PstI csoS4A_PstI-rev] were used:

Source

Gibson

References