Difference between revisions of "Part:BBa K1465101:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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BB
 
  
  
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*Amplification of the pSB1C3-Backbone (<bbpart>BBa_J04450</bbpart>) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_pccH pSB1C3_pre_pccH] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_pccH pSB1C3_suf_pccH]:<br>
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'''pSB1C3_pre_pccH (58 bp)'''<br>
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5'-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3'<br>
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'''pSB1C3_suf_pccH (43 bp)'''<br>
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5'-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3'<br>
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*Amplification of the cytochrome C (pccH) from ''Geobacter sulfurreducens KN400'' using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_fwd pccH_fwd] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_rev pccH_rev]:<br>
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'''pccH_fwd (55 bp)'''<br>
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5'-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3'<br>
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'''pccH_rev (40 bp)'''<br>
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5'-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3'<br>
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*[http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the cyctochrome c from ''Geobacter sulfurreducens'' containing also the RBS <bbpart>BBa_B0034</bbpart>.<br>
  
 
===Source===
 
===Source===

Revision as of 20:32, 3 October 2014


Cytochrome c from Geobacter sulfurreducens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 352
    Illegal SapI.rc site found at 433


Design Notes

  • Amplification of the pSB1C3-Backbone (BBa_J04450) using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_pre_pccH pSB1C3_pre_pccH] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pSB1C3_suf_pccH pSB1C3_suf_pccH]:
pSB1C3_pre_pccH (58 bp)

5'-GAAAGAATAGTTGTTTTTTGCATTTTCTCCTCTTTCTCTAGAAGCGGCCGCGAATTCC-3'

pSB1C3_suf_pccH (43 bp)

5'-CCATCACGGTCACGTACTGATACTAGTAGCGGCCGCTGCAGTC-3'
  • Amplification of the cytochrome C (pccH) from Geobacter sulfurreducens KN400 using the specific Gibson-Primer [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_fwd pccH_fwd] and [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Primer#pccH_rev pccH_rev]:
pccH_fwd (55 bp)

5'-ATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAAATGCAAAAAACAACTATTCTTTC-3'

pccH_rev (40 bp)

5'-TGCAGCGGCCGCTACTAGTATCAGTACGTGACCGTGATGG-3'
  • [http://2014.igem.org/Team:Bielefeld-CeBiTec/Notebook/Protocols#Gibson Gibson-Assembly] of the pSB1C3-Backbone and the cyctochrome c from Geobacter sulfurreducens containing also the RBS BBa_B0034.

Source

Gibson

References