Difference between revisions of "Part:BBa K592011"
Line 28: Line 28:
 
<partinfo>BBa_K592011 parameters</partinfo>
 
<partinfo>BBa_K592011 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
 +
===Structure and SWISS MODEL Homology Report===
 +
(The following information has been contributed by SVCE_Chennai 2016)
 +
 +
<!-- -->
 +
The following sections give a detailed information on this chromoprotein done by its in silico analysis.
 +
 +
 +
 +
 +
<!-- -->
 +
<span class='h3bb'><strong>3D Structure</strong></span>
 +
 +
[[Image:cjblueswiss.gif]]
 +
 +
 +
 +
 +
<!-- -->
 +
<span class='h3bb'><strong>Cartoon representation of cjBlue chromoprotein.</strong></span>
 +
 +
[[Image:cj blue swiss.png]]

Revision as of 13:03, 3 October 2016

cjBlue, green chromoprotein

This chromoprotein from the Cnidopus japonicus sea anemone, cjBlue, naturally exhibits dark green color when expressed. Compared to some other chromoproteins, such as amilCP (BBa_K592009), amilGFP (BBa_K592010), spisPink (BBa_K1033932), asPink (BBa_K1033933) and aeBlue (BBa_K864401), the color development is slower. The color is readily observed in both LB or on agar plates after 24-48 hours of incubation. The sequence is codon optimized for expression in E coli.

Usage and Biology

This part is useful as a reporter.

CjBlue plate small.jpg

iGEM11_Uppsala-Sweden: Expression of green chromoprotein. Escherichia coli constitutively expressing cjBlue (BBa_K592011).

UUChromo.jpg

iGEM12_Uppsala_University: The Uppsala chromoprotein collection and RFP. The image shows pellets of E coli expressing chromoproteins eforRed BBa_K592012, RFP BBa_E1010, cjBlue BBa_K592011, aeBlue BBa_K864401, amilGFP BBa_K592010 and amilCP BBa_K592009.

References

[http://www.jbc.org/content/281/49/37813.abstract] Chan MC, Karasawa S, Mizuno H, Bosanac I, Ho D, Privé GG, Miyawaki A, Ikura M. (2006) Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone Cnidopus japonicus. J. Biol. Chem. 281(49). 37813-9


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Structure and SWISS MODEL Homology Report

(The following information has been contributed by SVCE_Chennai 2016)

The following sections give a detailed information on this chromoprotein done by its in silico analysis.



3D Structure

Cjblueswiss.gif



Cartoon representation of cjBlue chromoprotein.

Cj blue swiss.png