Difference between revisions of "Part:BBa K1104200:Experience"
(→Applications of BBa_K1104200) |
|||
| Line 8: | Line 8: | ||
===Applications of BBa_K1104200=== | ===Applications of BBa_K1104200=== | ||
| + | [[Image:NYMU_O12.png|frame|center|'''OxyR-included circuit(Device1+Device2)''']] | ||
| + | |||
| + | In order to enhance the strength and sensitivity of ROS-sensing promoters, we make E. coli continuously produce inactivated OxyR, so that ROS can affect promoters more easily by adding a device: Device1. | ||
| + | |||
| + | Device1 in order to enhance the effect of ROS on ''E. coli'' is composed of : | ||
| + | #a constitutive promoter([https://parts.igem.org/Part:BBa_J23102 Part:BBa_J23102]) | ||
| + | #RBS([https://parts.igem.org/Part:BBa_B0034 Part:BBa_B0034]) | ||
| + | #activator OxyR([https://parts.igem.org/Part:BBa_K1104200 Part:BBa_K1104200]) | ||
| + | #double terminator [https://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015 | ||
| + | |||
| + | {| | ||
| + | |[[Image:NYMU_O1.png|frame|center|'''Device1'''<br>a constitutive promoter([https://parts.igem.org/Part:BBa_J23102 Part:BBa_J23102])+ RBS([https://parts.igem.org/Part:BBa_B0034 Part:BBa_B0034])+ activator OxyR([https://parts.igem.org/Part:BBa_K1104200 Part:BBa_K1104200])+ double terminator [https://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015]]] | ||
| + | |[[Image:NYMU_O2.png|frame|center|'''Device2'''<br>a sensor (OxyR-induced promoter, including TrxCp, HemHp, sufA, AhpCp1000, AhpCp2D1, AhpCp2, AhpCpD1, AhpCp1, DsbGp) + reporter ([https://parts.igem.org/Part:BBa_E0840 Part:BBa_E0840])]] | ||
| + | |} | ||
| + | |||
| + | Putting Device1 [https://parts.igem.org/Part:BBa_K1104250 BBa_K1104250] upstream to promoter AhpCp2D1([https://parts.igem.org/Part:BBa_K1104204 Part:BBa_K1104204]) remarkably enhanced the strength of promoter AhpCp2D1([https://parts.igem.org/Part:BBa_K1104204 Part:BBa_K1104204]) as our experiment results shows. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:38, 27 October 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
SDS-PAGE
We conduct an SDS Polyacrylamide gel electrophoresis for OxyR expression.
Setting control group contains only E.coli MG1655 and experimental group with K1104250 added to AhpCp2D1(K1104204), the electrophoresis result shows OxyR (Molecular Weight of Polypeptide: 34.276 kD) can really be expressed.
Applications of BBa_K1104200
In order to enhance the strength and sensitivity of ROS-sensing promoters, we make E. coli continuously produce inactivated OxyR, so that ROS can affect promoters more easily by adding a device: Device1.
Device1 in order to enhance the effect of ROS on E. coli is composed of :
- a constitutive promoter(Part:BBa_J23102)
- RBS(Part:BBa_B0034)
- activator OxyR(Part:BBa_K1104200)
- double terminator [https://parts.igem.org/Part:BBa_B0015 Part:BBa_B0015
 Device1 a constitutive promoter(Part:BBa_J23102)+ RBS(Part:BBa_B0034)+ activator OxyR(Part:BBa_K1104200)+ double terminator Part:BBa_B0015 |
 Device2 a sensor (OxyR-induced promoter, including TrxCp, HemHp, sufA, AhpCp1000, AhpCp2D1, AhpCp2, AhpCpD1, AhpCp1, DsbGp) + reporter (Part:BBa_E0840) |
Putting Device1 BBa_K1104250 upstream to promoter AhpCp2D1(Part:BBa_K1104204) remarkably enhanced the strength of promoter AhpCp2D1(Part:BBa_K1104204) as our experiment results shows.
User Reviews
UNIQ1465dd321ae05b60-partinfo-00000000-QINU UNIQ1465dd321ae05b60-partinfo-00000001-QINU