Difference between revisions of "Part:BBa K1031917"

 
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<partinfo>BBa_K1031917 short</partinfo>
 
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<p>For detailed information concerning XylS, please visit <a href="http://2013.igem.org/Team:Peking/Project/BioSensors/XylS">2013 Peking iGEM Biosensor XylS</a></p>
  
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117-XylS-TT
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===Usage and Biology===
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== '''Characterization''' ==
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In order to fine-tune the performance of XylS biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031917 is composed of three elements, the constitutive ''Pc'' promoter <html><a href="https://parts.igem.org/Part:BBa_J23105">J23117</a></html>, coding sequence of XylS and terminator <html><a href="https://parts.igem.org/Part:BBa_B0015">B0015</a></html>. ('''Fig 1''')
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<img src="https://static.igem.org/mediawiki/2013/thumb/d/d0/Peking2013_part_117-XylS_construction.png/800px-Peking2013_part_117-XylS_construction.png"
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<p style="text-align:center"><b>Fig 1</b> Construction of biosensor circuit <i>Pm</i>/J23117-XylS. Orange arrowheads represent promoters. RBSs are shown as green ovals and squares in dark red stand for B0015 terminator.
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== '''Sequence and Features''' ==
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1031917 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1031917 SequenceAndFeatures</partinfo>
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== '''Data shown''' ==
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Induction ratio of XylS biosensor library from ON/OFF test that utilizes different ''Pc'' promoters to control the expression of XylS. The dashed box refers to data for ''Pm''/J23117-XylS biosensor circuit ('''Fig 2''')
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<p style="text-align:center"><b>Fig 2</b> Horizontal axis stands for different XylS biosensor with <i>Pc</i> promoters of different strength. The expression strength of these constitutive promoters, J23113, J23109, J23114, J23105, J23106 is 21, 106, 256, 623, and 1185, respectively, according to the Parts' registry. Four kinds of aromatics, namely BzO, 2-MxeBzO, 3-MeBzO and 4-MeBzO, shown with different color intensities, were tested following <a href="http://2013.igem.org/Team:Peking/Team/Notebook/Protocols">Test Protocol 1</a>. Vertical axis represents the ON/OFF induction ratio. The dashed box refers to performance for <i>Pm</i>/J23117-XylS biosensor circuit.
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Revision as of 14:53, 18 October 2013

J23117-XylS-Terminator

For detailed information concerning XylS, please visit 2013 Peking iGEM Biosensor XylS


Characterization

In order to fine-tune the performance of XylS biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031917 is composed of three elements, the constitutive Pc promoter J23117, coding sequence of XylS and terminator B0015. (Fig 1)

Fig 1 Construction of biosensor circuit Pm/J23117-XylS. Orange arrowheads represent promoters. RBSs are shown as green ovals and squares in dark red stand for B0015 terminator.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 94
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 816
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 279


Data shown

Induction ratio of XylS biosensor library from ON/OFF test that utilizes different Pc promoters to control the expression of XylS. The dashed box refers to data for Pm/J23117-XylS biosensor circuit (Fig 2)

Fig 2 Horizontal axis stands for different XylS biosensor with Pc promoters of different strength. The expression strength of these constitutive promoters, J23113, J23109, J23114, J23105, J23106 is 21, 106, 256, 623, and 1185, respectively, according to the Parts' registry. Four kinds of aromatics, namely BzO, 2-MxeBzO, 3-MeBzO and 4-MeBzO, shown with different color intensities, were tested following Test Protocol 1. Vertical axis represents the ON/OFF induction ratio. The dashed box refers to performance for Pm/J23117-XylS biosensor circuit.