Difference between revisions of "Part:BBa K1067007"

Revision as of 12:48, 12 October 2013

Tight pBAD with araC

Non leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as part:BBa_K808000 but with a change i pBAD promoter and another sequence annotation. The promoter is taken from the pBAD SPL col. 10 from [http://2013.igem.org/Team:DTU-Denmark 2013 DTU iGEM team].

Characterization

Non-induced activity vs inducted activity for a variety of promoters built from the pBAD_SPL. This biobrick (BBa_K1067007) is built with colony 10. The diagonal line represents 1:1, on which the constitutive reference promoter sits. The horizontal blue line represents the induced activity of BBa_K808000.

For further characterization and information see the pBAD SPL page or go to the 2013 DTU [http://2013.igem.org/Team:DTU-Denmark/pBAD_SPL wiki].

Usage and Biology

Expression of lethal proteins
Tight control
Direct proteins to the periplasm, see Part:BBa_K1067009

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1182
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1182
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1182
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 4: / Line 4: @@
 Non leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as [[part:BBa_K808000]] but with a change i pBAD promoter and another sequence annotation. The promoter is taken from the [[pBAD SPL]] col. 10 from [http://2013.igem.org/Team:DTU-Denmark 2013 DTU iGEM team].
-For characterization see the [https://parts.igem.org/PBAD_SPL pBAD SPL page] or go to the 2013 DTU [http://2013.igem.org/Team:DTU-Denmark/pBAD_SPL wiki].
+===Characterization===
+[[File:690px-Induced vs basal.png|thumb|upright=3|center|Non-induced activity vs inducted activity for a variety of promoters built from the pBAD_SPL. This biobrick (BBa_K1067007) is built with colony 10.  The diagonal line represents 1:1, on which the constitutive reference promoter sits.  The horizontal blue line represents the induced activity of BBa_K808000.]]
+For further characterization and information see the [https://parts.igem.org/PBAD_SPL pBAD SPL page] or go to the 2013 DTU [http://2013.igem.org/Team:DTU-Denmark/pBAD_SPL wiki].