Difference between revisions of "Part:BBa K1067007"
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Non leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as [[part:BBa_K808000]] but with a change i pBAD promoter and another sequence annotation. The promoter is taken from the [[pBAD SPL]] col. 10 from [http://2013.igem.org/Team:DTU-Denmark 2013 DTU iGEM team].
 
Non leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as [[part:BBa_K808000]] but with a change i pBAD promoter and another sequence annotation. The promoter is taken from the [[pBAD SPL]] col. 10 from [http://2013.igem.org/Team:DTU-Denmark 2013 DTU iGEM team].
  
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For characterization see the [https://parts.igem.org/PBAD_SPL pBAD SPL page] or go to the 2013 DTU [http://2013.igem.org/Team:DTU-Denmark/pBAD_SPL wiki]
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For characterization see the [https://parts.igem.org/PBAD_SPL pBAD SPL page] or go to the 2013 DTU [http://2013.igem.org/Team:DTU-Denmark/pBAD_SPL wiki].
 
   
 
   
  

Revision as of 16:04, 4 October 2013

Tight pBAD with araC

Non leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as part:BBa_K808000 but with a change i pBAD promoter and another sequence annotation. The promoter is taken from the pBAD SPL col. 10 from [http://2013.igem.org/Team:DTU-Denmark 2013 DTU iGEM team].

For characterization see the pBAD SPL page or go to the 2013 DTU [http://2013.igem.org/Team:DTU-Denmark/pBAD_SPL wiki].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1182
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1182
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1182
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961