Difference between revisions of "PBAD SPL"

Revision as of 10:56, 4 October 2013

Here the DTU iGEM team will present their work on characterizing a SPL of pBAD promoters.

List of sequences:

DTU pBAD SPL

Identifier	Sequence^a	Induced Strength	Leakyness
Col. 2	CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA	14.2	0.203
Col. 3	CTGACGTAATCATACCGCCGAAAGTATTATCATTA	16.6	0.426
Col. 4	CTGACGCGACAACATTGCGTCCTATAAAATGCCGA	14.2	0.345
Col. 5	CTGACGGCCCGCCCCCGATGCGCATAAAATACCAA	17.7	0.424
Col. 12	CTGACGCGTATCGCGAGCGGGCGTTATTATACGCA	13.6	0.414
Col. 10	CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA	18	0.462
Col. 9	CTGACGCCACCCCCCCCCCCGGCGTATAATTCCCA	16.1	0.530
Col. 8	CTGACGCCCTACCGCTCGCCCCCCTATTATACCCA	13.4	0.211
Col. 13	CTGACGCCCCCCCCGTCCACCCCCTAATATCCCGA	10.9	0.096
Col. 15	CTGACGTGCGCCTGCCGTCCAAAGTAATATCCTTA	15.5	0.235
Col. 18	CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA	19.8	0.524
Col. 19	CTGACGCTCGCACCGGCGACCCGATAATATCCATA	18.0	0.315
Col. 31	CTGACGCCCCCCCTCCCCCGACCTAAAATATCCAG	3.8	0.161
Col. 29	CTGACGCCGCTGCACCCGTCCCCCTATTATAGCAA	14.8	0.301
Col. 26	CTGACGCCGATCCTATCTCCCTATTAAAATTTTTA	13.8	0.292
Col. 22	CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA	16.8	0.466
Col. 33	CTGACGCCAGCTTCCTACTTCAAATATAATCCGTA	14.4	1.01
Col. 34	CTGACGTCCCTCTTCCGCGCCCCCTAAAATACCCA	12.4	0.187
Col. 35	CTGACGCCCAACCCGACACAGCGATATAATAATGA	18.4	0.89
BBa_J23118	ttgacggctagctcagtcctaggtattgtgctagc	0.56

@@ Line 26: / Line 26: @@
 |align="center"|0.345
 |-
-|align="center"|<partinfo>J23102</partinfo>
+|align="center"|Col. 5
-|<font face="Courier" size="+1">ttgacagctagctcagtcctaggta<font color="red">c</font>t<font color="red">g</font>tgctagc</font>
+|<font face="Courier" size="+1">CTGACGGCCCGCCCCCGATGCGCATAAAATACCAA</font>
-|align="center"|0.86
+|align="center"|17.7
+|align="center"|0.424
 |-
-|align="center"|<partinfo>J23103</partinfo>
+|align="center"|Col. 12
-|<font face="Courier" size="+1"><font color="red">c</font>tga<font color="red">t</font>agctagctcagtcctagg<font color="red">g</font>attatgctagc</font>
+|<font face="Courier" size="+1">CTGACGCGTATCGCGAGCGGGCGTTATTATACGCA</font>
-|align="center"|0.01
+|align="center"|13.6
+|align="center"|0.414
 |-
-|align="center"|<partinfo>J23104</partinfo>
+|align="center"|Col. 10
-|<font face="Courier" size="+1">ttgacagctagctcagtcctaggtatt<font color="red">g</font>tgctagc</font>
+|<font face="Courier" size="+1">CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA</font>
-|align="center"|0.72
+|align="center"|18
+|align="center"|0.462
 |-
-|align="center"|<partinfo>J23105</partinfo>
+|align="center"|Col. 9
-|<font face="Courier" size="+1">tt<font color="red">t</font>ac<font color="red">g</font>gctagctcagtcctaggta<font color="red">c</font>tatgctagc</font>
+|<font face="Courier" size="+1">CTGACGCCACCCCCCCCCCCGGCGTATAATTCCCA</font>
-|align="center"|0.24
+|align="center"|16.1
+|align="center"|0.530
 |-
-|align="center"|<partinfo>J23106</partinfo>
+|align="center"|Col. 8
-|<font face="Courier" size="+1">tt<font color="red">t</font>ac<font color="red">g</font>gctagctcagtcctaggtat<font color="red">ag</font>tgctagc</font>
+|<font face="Courier" size="+1">CTGACGCCCTACCGCTCGCCCCCCTATTATACCCA</font>
-|align="center"|0.47
+|align="center"|13.4
+|align="center"|0.211
 |-
-|align="center"|<partinfo>J23107</partinfo>
+|align="center"|Col. 13
-|<font face="Courier" size="+1">tt<font color="red">t</font>ac<font color="red">g</font>gctagctcag<font color="red">c</font>cctaggtattatgctagc</font>
+|<font face="Courier" size="+1">CTGACGCCCCCCCCGTCCACCCCCTAATATCCCGA</font>
-|align="center"|0.36
+|align="center"|10.9
+|align="center"|0.096
 |-
-|align="center"|<partinfo>J23108</partinfo>
+|align="center"|Col. 15
-|<font face="Courier" size="+1"><font color="red">c</font>tgacagctagctcagtcctaggtat<font color="red">a</font>atgctagc</font>
+|<font face="Courier" size="+1">CTGACGTGCGCCTGCCGTCCAAAGTAATATCCTTA</font>
-|align="center"|0.51
+|align="center"|15.5
+|align="center"|0.235
 |-
-|align="center"|<partinfo>J23109</partinfo>
+|align="center"|Col. 18
-|<font face="Courier" size="+1">tt<font color="red">t</font>acagctagctcagtcctagg<font color="red">g</font>a<font color="red">ctg</font>tgctagc</font>
+|<font face="Courier" size="+1">CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA</font>
-|align="center"|0.04
+|align="center"|19.8
+|align="center"|0.524
 |-
-|align="center"|<partinfo>J23110</partinfo>
+|align="center"|Col. 19
-|<font face="Courier" size="+1">tt<font color="red">t</font>ac<font color="red">g</font>gctagctcagtcctaggta<font color="red">ca</font>atgctagc</font>
+|<font face="Courier" size="+1">CTGACGCTCGCACCGGCGACCCGATAATATCCATA</font>
-|align="center"|0.33
+|align="center"|18.0
+|align="center"|0.315
 |-
-|align="center"|<partinfo>J23111</partinfo>
+|align="center"|Col. 31
-|<font face="Courier" size="+1">ttgac<font color="red">g</font>gctagctcagtcctaggtat<font color="red">ag</font>tgctagc</font>
+|<font face="Courier" size="+1">CTGACGCCCCCCCTCCCCCGACCTAAAATATCCAG</font>
-|align="center"|0.58
+|align="center"|3.8
+|align="center"|0.161
 |-
-|align="center"|<partinfo>J23112</partinfo>
+|align="center"|Col. 29
-|<font face="Courier" size="+1"><font color="red">c</font>tga<font color="red">t</font>agctagctcagtcctagg<font color="red">g</font>attatgctagc</font>
+|<font face="Courier" size="+1">CTGACGCCGCTGCACCCGTCCCCCTATTATAGCAA</font>
-|align="center"|0.00
+|align="center"|14.8
+|align="center"|0.301
 |-
-|align="center"|<partinfo>J23113</partinfo>
+|align="center"|Col. 26
-|<font face="Courier" size="+1"><font color="red">c</font>tga<font color="red">tg</font>gctagctcagtcctagg<font color="red">g</font>attatgctagc</font>
+|<font face="Courier" size="+1">CTGACGCCGATCCTATCTCCCTATTAAAATTTTTA</font>
-|align="center"|0.01
+|align="center"|13.8
+|align="center"|0.292
 |-
-|align="center"|<partinfo>J23114</partinfo>
+|align="center"|Col. 22
-|<font face="Courier" size="+1">tt<font color="red">t</font>a<font color="red">tg</font>gctagctcagtcctaggta<font color="red">ca</font>atgctagc</font>
+|<font face="Courier" size="+1">CTGACGCCCCGAATCAGTAGTATTTATTATCTAGA</font>
-|align="center"|0.10
+|align="center"|16.8
+|align="center"|0.466
 |-
-|align="center"|<partinfo>J23115</partinfo>
+|align="center"|Col. 33
-|<font face="Courier" size="+1">tt<font color="red">t</font>a<font color="red">t</font>agctagctcag<font color="red">c</font>cct<font color="red">t</font>ggta<font color="red">ca</font>atgctagc</font>
+|<font face="Courier" size="+1">CTGACGCCAGCTTCCTACTTCAAATATAATCCGTA</font>
-|align="center"|0.15
+|align="center"|14.4
+|align="center"|1.01
 |-
-|align="center"|<partinfo>J23116</partinfo>
+|align="center"|Col. 34
-|<font face="Courier" size="+1">ttgacagctagctcagtcctagg<font color="red">g</font>a<font color="red">c</font>tatgctagc</font>
+|<font face="Courier" size="+1">CTGACGTCCCTCTTCCGCGCCCCCTAAAATACCCA</font>
-|align="center"|0.16
+|align="center"|12.4
+|align="center"|0.187
 |-
-|align="center"|<partinfo>J23117</partinfo>
+|align="center"|Col. 35
-|<font face="Courier" size="+1">ttgacagctagctcagtcctagg<font color="red">g</font>att<font color="red">g</font>tgctagc</font>
+|<font face="Courier" size="+1">CTGACGCCCAACCCGACACAGCGATATAATAATGA</font>
-|align="center"|0.06
+|align="center"|18.4
+|align="center"|0.89
 |-
 |align="center"|<partinfo>J23118</partinfo>