Difference between revisions of "Part:BBa K1067007"
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<partinfo>BBa_K1067007 short</partinfo> | <partinfo>BBa_K1067007 short</partinfo> | ||
+ | No leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as [[part:BBa_K808000]] but with a change i pBAD promoter and another sequence annotation. The promoter is engineered as part of the [[pBAD SPL]] project from DTU iGEM team 2013. | ||
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Revision as of 17:43, 28 September 2013
Tight pBAD with araC No leaky pBAD promoter with araC constitutively expressed. This biobrick is the same as part:BBa_K808000 but with a change i pBAD promoter and another sequence annotation. The promoter is engineered as part of the pBAD SPL project from DTU iGEM team 2013.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1182
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1182
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1182
Illegal AgeI site found at 979 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961