Difference between revisions of "Part:BBa K1065306:Design"
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===Source=== | ===Source=== | ||
| − | This part has been built using | + | This part has been built using:<br> |
<partinfo>Bba_J23100</partinfo> a constitutive promoter from the Anderson family.<br> | <partinfo>Bba_J23100</partinfo> a constitutive promoter from the Anderson family.<br> | ||
<partinfo>Bba_K592016</partinfo> the part with RBS-coupled YF1 and FixJ. | <partinfo>Bba_K592016</partinfo> the part with RBS-coupled YF1 and FixJ. | ||
Revision as of 10:13, 28 September 2013
J23100+YF1+FixJ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 605
Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 767
Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 1590
Illegal NgoMIV site found at 1684
Illegal AgeI site found at 319
Illegal AgeI site found at 1465 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1354
Illegal BsaI.rc site found at 218
Design Notes
This part was built by starting from the extraction of BBa_K592016 from the iGEM Registry 2013 Kit Distribution, then it was subcloned in BBa_J61002 by standard assembly.
Source
This part has been built using:
BBa_J23100 a constitutive promoter from the Anderson family.
BBa_K592016 the part with RBS-coupled YF1 and FixJ.
References
1. Andreas Möglich, Rebecca A. Ayers, Keith Moffat (2009). Design and Signaling Mechanism of Light-Regulated Histidine Kinases. Journal of Molecular Biology, 1433–1444.