Difference between revisions of "Part:BBa K1124106:Design"
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===Design Notes===
 
===Design Notes===
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In this composite part, we chose the phage lambda pR promoter([[Part:BBa_R0051]]) as the promoter of sRNA because the transcriptional start site of pR promoter was well determined, and its operator site is embedded within the pR promoter. These characteristics  
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In this composite part, we chose the phage lambda pR promoter([[Part:BBa_R0051]]) as the promoter of sRNA because the transcriptional start site of pR promoter was well determined, and its operator site is embedded within the pR promoter. These characteristics strongly suggest that no dummy nucleotides will be attached to the sRNA.
  
  
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===References===
 
===References===
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Yoo, S. M., Na, D., & Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocols, 8(9), 1694-1707.

Revision as of 23:58, 27 September 2013

plambda-sRNA(anti-tyrR)-plambda-sRNA (anti-csrA) (tyrosine synthesis device)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In this composite part, we chose the phage lambda pR promoter(Part:BBa_R0051) as the promoter of sRNA because the transcriptional start site of pR promoter was well determined, and its operator site is embedded within the pR promoter. These characteristics strongly suggest that no dummy nucleotides will be attached to the sRNA.


Source

Coming soon!

References

Yoo, S. M., Na, D., & Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocols, 8(9), 1694-1707.