Difference between revisions of "Part:BBa K1189018"
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<partinfo>BBa_K1189018 short</partinfo>
 
<partinfo>BBa_K1189018 short</partinfo>
  
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<p>This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART). </p>
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<p>This part was created by fusing the heavy chain and light chains (<partinfo>BBa_K1189024</partinfo> <partinfo>BBa_K1189025</partinfo>) of human ferritin together. It is expressed under the lacI promoter (<partinfo>BBa_J04500</partinfo>) and has a his-tag for protein purification. An E-coil (<partinfo>BBa_K1189011</partinfo>) is included in order to allow binding of parts containing the respective K-coil (<partinfo>BBa_K1189010</partinfo>). </p>
 
<p>This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.</p>
 
<p>This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.</p>
 
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Revision as of 08:02, 27 September 2013

Human ferritin di-subunit with E coil w/ LacI promoter

This part was created by fusing the heavy chain and light chains (BBa_K1189024 BBa_K1189025) of human ferritin together. It is expressed under the lacI promoter (BBa_J04500) and has a his-tag for protein purification. An E-coil (BBa_K1189011) is included in order to allow binding of parts containing the respective K-coil (BBa_K1189010).

This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.


UCalgary2013TRPrussianblueferritinsynthesispartspage.png

Figure 4. Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period.


The resulting molecuel can then catalyse the formation of radicals from hydrogen perxoide, which can then cause a colour change in substrates such as TMB or ABTS.
UCalgary2013TRSubstratecolourpartspage.png
<p> Figure 11. Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.


Something about scaffold…


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1289