Difference between revisions of "Part:BBa K1189020"

Revision as of 07:49, 27 September 2013

Light chain human ferritin with his-tag and E-coil under the lacI inducible promoter

This light ferritin chain comes from humans. This part along with heavy ferritin (BBa_K1189025), form the ferritin nanoparticle, an iron-storage particle made up of 24 subunits. The formed nanoparticle is highly robust, remaining stable at extreme pHs and temperatures.

This nanoparticle can also be used as a reporter when the iron core is modified with potassium ferrocyanide to form Prussian Blue. The Prussian Blue ferritin can then act as a peroxidase mimic, similar to horseradish peroxidase, resulting in colour changes in the presence of hydrogen peroxide, and TMB or ABTS.

We added the lacI promoter (BBa_J04500), double terminator (BBa_B0010, & BBa_B0012) and a his-tag in order for us to induce protein expression as well as purify it.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 230
Illegal AgeI site found at 881
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 746

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1189020 short</partinfo>
-<p>This light ferritin chain comes from humans. This part along with heavy ferritin (<partinfo>BBa_K1189025</partinfo>), form the ferritin nanoparticle, an iron-storage particle made up of 24 subunits. The formed nanoparticle is highly robust, remaining stable at extreme pHs and temperatures. The difference between light ferritin is that this chain contains a ferroxidase centre.
+<p>This light ferritin chain comes from humans. This part along with heavy ferritin (<partinfo>BBa_K1189025</partinfo>), form the ferritin nanoparticle, an iron-storage particle made up of 24 subunits. The formed nanoparticle is highly robust, remaining stable at extreme pHs and temperatures.
 </p>
 <p>