Difference between revisions of "Part:BBa K1073020:Experience"

Revision as of 19:49, 26 September 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1073020

When aeBlue is expressed, the colonies on plates as well as the liquid culture become blue. The iGEM Team Braunschweig 2013 used this device to differentiate two different strains from one approach.

User Reviews

UNIQ6e6aff9dd9a1cad8-partinfo-00000000-QINU

•••••

iGEM Team Braunschweig 2013

The chromoprotein aeBlue exhibits a distinct blue color when expressed. In order to avoid absoption of the chomoproteins during measurement with the photometer the absorption and emission spectra were measured.

iGEM Team Braunschweig 2013: Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm.

iGEM Team Braunschweig 2013:Emission spectrum of chromoprotein aeBlue. This chromoprotein does not show any significant emission in a wavelength range between 500-800 nm and therefore cannot be detected by its fluorescence.

Absorption maximum for aeBlue: 595 nm

Emission maximum for aeBlue: none (dark quencher)

Accordingly the photometer measurements for growth curves were carried out by 520 nm with respect to the chromoproteins amilGFP (BBa_K1073024) and eforRed (BBa_K1073022).

;

UNIQ6e6aff9dd9a1cad8-partinfo-00000004-QINU

@@ Line 20: / Line 20: @@
 [[File:AeBlue.jpg |500px]]
-'''Braunschweig 2013:''' Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm.
+'''iGEM Team Braunschweig 2013:''' Absorption spectrum of chromoprotein aeBlue in a wavelength range of 400-800 nm.
+[[File:BS2013Emission_aeBlue.jpg |500px]]
+'''iGEM Team Braunschweig 2013:'''Emission spectrum of chromoprotein aeBlue. This chromoprotein does not show any significant emission in a wavelength range between  500-800 nm and therefore cannot be detected by its fluorescence.
 Absorption maximum for aeBlue: 595 nm