Difference between revisions of "PBAD SPL"
Line 9: Line 9:
 
! colspan="2" style="background:#AAAAAA; color:black"  |Measured Strength<sup>b</sup>
 
! colspan="2" style="background:#AAAAAA; color:black"  |Measured Strength<sup>b</sup>
 
|-
 
|-
−
|align="center"|Mut. 1
+
|align="center"|Col. 2
−
|<font face="Courier" size="+1">ttgacagctagctcagtcctaggtataatgctagc</font>
+
|<font face="Courier" size="+1">CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA</font>
−
|align="center"|n/a
+
|align="center"|14.2
 
|-
 
|-
−
|align="center"|Mut. 2
+
|align="center"|Col. 3
−
|<font face="Courier" size="+1">ttgac<font color="red">g</font>gctagctcagtcctaggta<font color="red">c</font>a<font color="red">g</font>tgctagc</font>
+
|<font face="Courier" size="+1">CTGACGTAATCATACCGCCGAAAGTATTATCATTA</font>
−
|align="center"|1
+
|align="center"|16.6
 
|-
 
|-
−
|align="center"|<partinfo>J23101</partinfo>
+
|align="center"|Col. 4
−
|<font face="Courier" size="+1">tt<font color="red">t</font>acagctagctcagtcctaggtattatgctagc</font>
+
|<font face="Courier" size="+1">CTGACGCGACAACATTGCGTCCTATAAAATGCCGA</font>
−
|align="center"|0.70
+
|align="center"|14.2
 
|-
 
|-
 
|align="center"|<partinfo>J23102</partinfo>
 
|align="center"|<partinfo>J23102</partinfo>

Revision as of 10:29, 4 October 2013

Here the DTU iGEM team will present their work on characterizing a SPL of pBAD promoters.

List of sequences:

DTU pBAD SPL

Identifier Sequencea Measured Strengthb
Col. 2 CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA 14.2
Col. 3 CTGACGTAATCATACCGCCGAAAGTATTATCATTA 16.6
Col. 4 CTGACGCGACAACATTGCGTCCTATAAAATGCCGA 14.2
BBa_J23102 ttgacagctagctcagtcctaggtactgtgctagc 0.86
BBa_J23103 ctgatagctagctcagtcctagggattatgctagc 0.01
BBa_J23104 ttgacagctagctcagtcctaggtattgtgctagc 0.72
BBa_J23105 tttacggctagctcagtcctaggtactatgctagc 0.24
BBa_J23106 tttacggctagctcagtcctaggtatagtgctagc 0.47
BBa_J23107 tttacggctagctcagccctaggtattatgctagc 0.36
BBa_J23108 ctgacagctagctcagtcctaggtataatgctagc 0.51
BBa_J23109 tttacagctagctcagtcctagggactgtgctagc 0.04
BBa_J23110 tttacggctagctcagtcctaggtacaatgctagc 0.33
BBa_J23111 ttgacggctagctcagtcctaggtatagtgctagc 0.58
BBa_J23112 ctgatagctagctcagtcctagggattatgctagc 0.00
BBa_J23113 ctgatggctagctcagtcctagggattatgctagc 0.01
BBa_J23114 tttatggctagctcagtcctaggtacaatgctagc 0.10
BBa_J23115 tttatagctagctcagcccttggtacaatgctagc 0.15
BBa_J23116 ttgacagctagctcagtcctagggactatgctagc 0.16
BBa_J23117 ttgacagctagctcagtcctagggattgtgctagc 0.06
BBa_J23118 ttgacggctagctcagtcctaggtattgtgctagc 0.56





Mutant name Sequence
Mut. 1 CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA
Mut. 2 CTGACGTAATCATACCGCCGAAAGTATTATCATTA
Mut. 3 CTGACGCGACAACATTGCGTCCTATAAAATGCCGA