Difference between revisions of "Part:BBa K1150011"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1150011 short</partinfo>
 
<partinfo>BBa_K1150011 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|SV40
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|-
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|'''Function'''
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|promotor
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|-
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|'''Use in'''
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|Mammalian cells
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10]
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Submitted by'''
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|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
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|}
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SV40 promotor is a viral promotor derived from Simian virus 40.  
 
SV40 promotor is a viral promotor derived from Simian virus 40.  

Revision as of 09:15, 27 September 2013

SV40 promoter

SV40
Function promotor
Use in Mammalian cells
RFC standard RFC 10
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]


SV40 promotor is a viral promotor derived from Simian virus 40.

Because we could not found the SV40 promotor in the parts registry, we decided to provide one to the following iGEM teams. This enables the teams to choose from differently strong mammalian promotors – dependent of the individual intention. We tested both promotors (BBa_K1150011 [SV40], BBa_K747096 [CMV]) with our own devices for expression rate and effectivity. For further information please take a look at BBa_K1150019 and BBa_K1150020.

In an earlier study CMV and SV40 promoter were tested in different B and T cell as well as in two non-lymphoid cell. In all cell lines the CMV promoter construct was higher expressed than that of SV40 [Zarrin et al., 1999].

In comparison to the cellular promoter VV1 SV40 showed similar activities. Hence with an SV40 promotor one can gain a more physiologic control for reporter assays compared to CMV.


ZARRIN, A.; MALKIN, L.; FONG, I.; LUK, K.; GHOSE, A.; BERINSTEIN, N. (1999). Comparison of CMV, RSV, SV40 viral and VV1 cellular promoters in B and T lymphoid and non-lymphoid cell lines. Biochimica et Biophysica Acta 1446: 135-139.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]