Difference between revisions of "Part:BBa R0011"

Revision as of 19:40, 17 July 2006

Promoter (lacI regulated, lambda pL hybrid)

Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be:

• repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]).
• induced by [http://openwetware.org/wiki/IPTG IPTG] in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range

Usage and Biology

Strong promoter. [jb, 5/24/04]

R0011 will be on in strains without lacI, off in strains that are lacIq (ie. Part:BBa_V1003) and medium in strains that are lacI

Sequence and Features