Difference between revisions of "Part:BBa K1031742"
Line 6: Line 6:
  
 
== '''Construction''' ==
 
== '''Construction''' ==
−
 
  
 
In order to fine-tune the performance of PaaX biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031742 is composed of three elements, the constitutive ''Pc'' promoter J23109[https://parts.igem.org/Part:BBa_J23109], coding sequence of PaaX and terminator B0015[https://parts.igem.org/Part:BBa_B0015]. ('''Fig 1''')
 
In order to fine-tune the performance of PaaX biosensor, we constructed a library of ''Pc'' constitutive promoters at different intensity. BBa_K1031742 is composed of three elements, the constitutive ''Pc'' promoter J23109[https://parts.igem.org/Part:BBa_J23109], coding sequence of PaaX and terminator B0015[https://parts.igem.org/Part:BBa_B0015]. ('''Fig 1''')
Line 14: Line 13:
 
</html>
 
</html>
  
−
'''Fig 1''' Construction of biosensor circuit ''Pa''/J23109-PaaX
+
'''Fig 1''' Construction of biosensor circuit.
 +
 
  
  

Revision as of 14:36, 26 September 2013

J23109-PaaX


Construction

In order to fine-tune the performance of PaaX biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031742 is composed of three elements, the constitutive Pc promoter J23109[1], coding sequence of PaaX and terminator B0015[2]. (Fig 1)

Fig 1 Construction of biosensor circuit.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 344
    Illegal NgoMIV site found at 625
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 851
    Illegal SapI.rc site found at 1508