Difference between revisions of "Part:BBa K817013"
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<partinfo>BBa_K817013 short</partinfo>
 
<partinfo>BBa_K817013 short</partinfo>
  
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As we sought to design a thermal sensitive composite part, we integrate our modified pCI promoter(modification of pCI, BBa_R0051) with temperature sensitive CI repressor, which is cloned from Harvard’s thermal regulation part, 2008. (BBa_K098988). We showed the modified promoter design had superior repression effects.  
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As we sought to design a thermal sensitive composite part, we integrate our modified pCI promoter(modification of pCI, [[Part:BBa_R0051|BBa_R0051]]) with temperature sensitive CI repressor, which is cloned from Harvard’s thermal regulation part, 2008. ([[Part:BBa_K098988|BBa_K098988]]). We showed the modified promoter design had superior repression effects.  
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===Usage and Biology===
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Revision as of 12:12, 5 October 2012

pCI(OR1-OL1)-mRFP

As we sought to design a thermal sensitive composite part, we integrate our modified pCI promoter(modification of pCI, BBa_R0051) with temperature sensitive CI repressor, which is cloned from Harvard’s thermal regulation part, 2008. (BBa_K098988). We showed the modified promoter design had superior repression effects.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 625
    Illegal AgeI site found at 737
  • 1000
    COMPATIBLE WITH RFC[1000]