Difference between revisions of "Part:BBa J45001"

Revision as of 21:37, 9 March 2008

SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter

BBa_J45001 encodes SAM salicylic acid carboxyl methyltransferase I derived from SAMT from Antirrhinus majus (snapdragon). SAMT catalyzes the conversion of salicylic acid to methyl salicylate. Methyl salicylate has a wintergreen smell.

Usage and Biology

The culture medium of E. coli cells expressing SAMT contained methyl salicylate (2.1 μg/ml) when the growing medium was supplemented with 5 μg/ml salicylic acid, and methyl benzoate (0.86 μg/ml) when the growing medium was supplemented with 5 μg/ml benzoic acid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 577
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 901
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 421
Illegal NgoMIV site found at 425
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 471
Illegal SapI.rc site found at 75

Functional Parameters

ec_num	none
kegg	none
protein	SAMT
swisspro	Q8H6N2

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J45001 short</partinfo>
-#From snapdragon (A. Majus)
+<partinfo>BBa_J45001</partinfo> encodes SAM salicylic acid carboxyl methyltransferase I derived from ''SAMT'' from ''Antirrhinus majus'' (snapdragon). SAMT catalyzes the conversion of salicylic acid to methyl salicylate.  Methyl salicylate has a wintergreen smell.
-#This carboxyl methyltransferase gene encodes an enzyme that catalyzes the transfer of the methyl group of SAM to the carboxyl group of salicylic acid to make the volatile ester methyl salicylate
 ===Usage and Biology===
-#The culture medium of the E. coli cells expressing SAMT contained methyl salicylate (2.1 μg/ml) when the growing medium was supplemented with 5 μg/ml salicylic acid, and methyl benzoate (0.86 μg/ml) when the growing medium was supplemented with 5 μg/ml benzoic acid.
+#The culture medium of ''E. coli'' cells expressing ''SAMT'' contained methyl salicylate (2.1 &mu;g/ml) when the growing medium was supplemented with 5 &mu;g/ml salicylic acid, and methyl benzoate (0.86 &mu;g/ml) when the growing medium was supplemented with 5 &mu;g/ml benzoic acid.
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