Difference between revisions of "Part:BBa K801065:Design"
(→Design Notes) |
Nadine1990 (Talk | contribs) |
||
Line 13: | Line 13: | ||
<br>'''Keywords:''' | <br>'''Keywords:''' | ||
<!--These keywords are necessary to find your part using a fulltext sarch.--> | <!--These keywords are necessary to find your part using a fulltext sarch.--> | ||
− | + | limonen, strep-tag | |
<br>'''Abbreviations:''' | <br>'''Abbreviations:''' | ||
Line 42: | Line 42: | ||
'''Backbone:'''<br> | '''Backbone:'''<br> | ||
− | + | *Backbone name: pSB1C3' | |
<!--*Resistance: Amp/Cp/Kan/Tet--> | <!--*Resistance: Amp/Cp/Kan/Tet--> | ||
<!--*Copynumber: low/medium/high--> | <!--*Copynumber: low/medium/high--> | ||
Line 53: | Line 53: | ||
'''Enzymatic activity:''' | '''Enzymatic activity:''' | ||
− | + | EC-number 4.2.3.20 | |
'''Cytotoxicity:'''<br> | '''Cytotoxicity:'''<br> | ||
Line 82: | Line 82: | ||
'''Organism:'''<br> | '''Organism:'''<br> | ||
− | <!--*Genesequence derived from '' | + | <!--*Genesequence derived from ''citrus limonen''--> |
<!--*Codonoptimized for ''?organism_name?''--> | <!--*Codonoptimized for ''?organism_name?''--> | ||
<!--*Designed for the following Chassis: ''?organism-name?''--> | <!--*Designed for the following Chassis: ''?organism-name?''--> |
Revision as of 15:31, 3 October 2012
(+)-limonene synthase 1 with Strep-Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1676
Illegal SapI.rc site found at 100
C-Terminal Strep-Tag added
See BBa_K801061
Keywords:
limonen, strep-tag
Abbreviations:
Design Notes
Related BioBrick:
- BBa_K801060: (+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence
- BBa_K801061: (+)-Limonene synthase 1 coding region
- BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression
Quality control measures:
Backbone:
- Backbone name: pSB1C3'
Protein coding:
Enzymatic activity: EC-number 4.2.3.20
Cytotoxicity:
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
Organism:
References
Literature references:
Database references: