Difference between revisions of "Part:BBa K901009"
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<partinfo>BBa_K901009 parameters</partinfo> | <partinfo>BBa_K901009 parameters</partinfo> | ||
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| + | ==Qdai 2022's Characterization== | ||
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| + | This transporter contributes to raising the pH of the culture fluid. In our experiments, this part was assembled to E.Coli, BL21 strains with poxB (<partinfo>BBa_K4206001</partinfo>) , which catalyzes the decarboxylation of pyruvate to make acetate ion and CO2. The genetically modified E.coli are cultured and induced with IPTG. After 12 or more hours, we measured the pH of the culture fluid based on the pH test paper. The measurement result was pH 8-9, both with and without IPTG induction. Therefore, this transporter transports acetate ions, whether the ion indicating alkalinity is abundant or not in the cell. Considering the growth limits of E.coli, this transporter needs attention. | ||
Revision as of 11:31, 11 October 2022
AatA from Acetobacter acetii
An ABC transporter for the acetogenic bacteria Acetobacter acetii. This transporter binds ATP and moves acetate across the membrane out of the cell. The protein has been shown to confer resistance to acetic acid.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Qdai 2022's Characterization
This transporter contributes to raising the pH of the culture fluid. In our experiments, this part was assembled to E.Coli, BL21 strains with poxB (BBa_K4206001) , which catalyzes the decarboxylation of pyruvate to make acetate ion and CO2. The genetically modified E.coli are cultured and induced with IPTG. After 12 or more hours, we measured the pH of the culture fluid based on the pH test paper. The measurement result was pH 8-9, both with and without IPTG induction. Therefore, this transporter transports acetate ions, whether the ion indicating alkalinity is abundant or not in the cell. Considering the growth limits of E.coli, this transporter needs attention.