Device

Part:BBa_K4206001

Designed by: Kazuma Tsurumaki   Group: iGEM22_Qdai   (2022-10-02)


poxB-aatA, acetate production

This part is placed downstream of the lac promoter of a plasmid. poxB, acetate production enzyme, is induced by IPTG. aatA, coding sequence of transporter of acetate, expresses on downstream of constitutive promoter. Acetate is transported out of the cell and acetate synthesis is promoted for more efficient acetate production

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1870
    Illegal NheI site found at 1893
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 352
    Illegal SapI.rc site found at 1600


Qdai_2023's Characterization

[[1]]

Usage and Biology

The iGEM Qdai team We used the promoter, RBS, and terminator structures of this part as is, changing poxB to Pdr12 and attA to AlaDH.And incorporated by Gibson Assembly into pTWV228. 

Experimental Results

Regardless of the concentration of alanine and incubation time, no changes in the concentration and absorbance of pyruvic acid were observed. This suggests that the amino acid transamination reaction may not be proceeding as expected, or that the changes are at a level undetectable within the range of our measurements.

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