Difference between revisions of "Part:BBa K864445"
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Due to technical limitations, the correct sequence can not be entered into the registry. The entered sequence should be preceded by 10 N:s, to denote the randomized region, and thus read: | Due to technical limitations, the correct sequence can not be entered into the registry. The entered sequence should be preceded by 10 N:s, to denote the randomized region, and thus read: | ||
| − | + | NNNNNNNNNN<b>ATTTGGCTGAATATTTTAG | |
| + | |||
| + | Tm1 = 51,7°C | ||
| + | |||
| + | Tm2 = 67.7°C | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 21:38, 29 September 2012
Spot42 randomized forward primer
This part is a component of the Uppsala University iGEM 2012 modular small RNA screening system.
This primer is used to create a random sRNA library, together with BBa_K864446, by randomizing the mRNA-binding region of the spot42 small RNA (BBa_K864444). This library can be used to screen for synthetic silencing sRNA:s.
Note
Due to technical limitations, the correct sequence can not be entered into the registry. The entered sequence should be preceded by 10 N:s, to denote the randomized region, and thus read:
NNNNNNNNNNATTTGGCTGAATATTTTAG
Tm1 = 51,7°C
Tm2 = 67.7°C
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]