Primer

Part:BBa_K864446

Designed by: Arvid Hedén Gynnå, Sabri Jamal, Lisa Branzell, Hampus Elofsson   Group: iGEM12_Uppsala   (2012-09-29)

Spot42 randomized reverse primer


This part is a component of the Uppsala University iGEM 2012 modular small RNA screening system.

This primer is used to create a random sRNA library, together with BBa_K864445, by randomizing the mRNA-binding region of the spot42 small RNA (BBa_K864444). This library can be used to screen for synthetic silencing sRNA:s.

Note

Due to technical limitations, the correct sequence can not be entered into the registry. The entered sequence should be preceded by 20 N:s, to denote the randomized region, and thus read:

NNNNNNNNNNNNNNNNNNNNGCTAGCATAATACCTAGGAC

Tm = 52,9°C

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

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Categories
Parameters
None