Difference between revisions of "Part:BBa K592010"

Revision as of 20:08, 27 September 2012

amilGFP, yellow chromoprotein

This chromoprotein from the coral Acropora millepora, amilGFP, naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation. The DNA was provided by Jeffrey Miller at UCLA, and was made BioBrick-compatible after removal of two illegal internal restriction sites (EcoRI and PstI).

Usage and Biology

This part is useful as a reporter.

iGEM11_Uppsala-Sweden: Expression of chromoproteins. The images above show E coli constitutively expressing amilGFP BBa_K592010 (yellow), amilCP BBa_K592009 (blue), and RFP BBa_E1010 (red). The Uppsala chromoprotein collection and RFP.

References

[http://www.ncbi.nlm.nih.gov/pubmed/18648549] Alieva, N. O., et al. 2008. Diversity and evolution of coral fluorescent proteins. PLoS One 3:e2680.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 [[Image:On cultures BYR small.jpg|300px]]
 [[Image:Pellets BYR.jpg|300px]]
-[[Image:UUChromo.jpg|900px]]
 '''iGEM11_Uppsala-Sweden:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing amilGFP BBa_K592010 (yellow), amilCP <partinfo>BBa_K592009</partinfo> (blue), and RFP <partinfo>BBa_E1010</partinfo> (red).
+[[Image:UUChromo.jpg|600px]]
+The Uppsala chromoprotein collection and RFP.
 ===References===