Difference between revisions of "Part:BBa K895007:Experience"

Revision as of 21:37, 26 September 2012

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Applications of BBa_K895007

Results

E. coli MG1655 was transformed with BBa_K895007 grown aerobically overnight in LB medium. The culture was then used to inoculate 30 ml anaerobic cultures in LB only, LB + 0.4% (w/v) tetrathionate or LB + 0.4% (w/v) thiosulfate. Following 16 hours growth aliquotes were taken and analysed by SDS-PAGE and Western immunoblotting using an anti-GFP monoclonal.

This small scale aassay reveals that GFP was only expressed from the plasmid in the presence of tetrathionate. The device therefore works as a putative inflammation biosensor.

Figure 1: Production of GFP from BBa_K895007 is tetrathionate dependent. The RED ARROW points to the lane producing GFP.

User Reviews

UNIQ79b8421ad5ba0da7-partinfo-00000002-QINU UNIQ79b8421ad5ba0da7-partinfo-00000003-QINU

@@ Line 4: / Line 4: @@
 ===Applications of BBa_K895007===
+===Results===
+<i>E. coli</i> MG1655 was transformed with <partinfo>BBa_K895007</partinfo> grown aerobically overnight in LB medium. The culture was then used to inoculate 30 ml anaerobic cultures in LB only, LB + 0.4% (w/v) tetrathionate or LB + 0.4% (w/v) thiosulfate. Following 16 hours growth aliquotes were taken and analysed by SDS-PAGE and Western immunoblotting using an anti-GFP monoclonal.
+This small scale aassay reveals that GFP was only expressed from the plasmid in the presence of tetrathionate. The device therefore works as a putative <b>inflammation biosensor</b>.
+[[Image:Dundee_Biosensor_Figure5.jpg]]
+:Figure 1: Production of GFP from <partinfo>BBa_K895007</partinfo> is tetrathionate dependent. The RED ARROW points to the lane producing GFP.
 ===User Reviews===