Difference between revisions of "Part:BBa K784046:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
−
Fusion PCR of [https://parts.igem.org/Part:BBa_K784005 BBa K784005] and [https://parts.igem.org/Part:BBa_K784044 K784044]
+
Assembly PCR of [https://parts.igem.org/Part:BBa_K784005 BBa K784005] and [https://parts.igem.org/Part:BBa_K784044 K784044]
  
 
===References===
 
===References===

Revision as of 08:42, 26 September 2012

theophylline riboswitch +T7*(N4) RNAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The riboswitch had to fused to the RNAP using assembly PCR.

Source

Assembly PCR of BBa K784005 and K784044

References