Difference between revisions of "Part:BBa K784046"
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− | This part contains an engineered theophylline riboswitch (RS) ([https://parts.igem.org/Part:BBa_K784005 BBa K784005]) and an engineered N4 RNA polymerase ([https://parts.igem.org/Part:BBa_K784044 K784044]). The riboswitch contains a RBS and it will be exposed and starts translating the N4 RNAP only after inserting of theophylline. The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the N4 RNAP are adjacent to one another without any restriction site between them. | + | This part contains an engineered theophylline riboswitch (RS) ([https://parts.igem.org/Part:BBa_K784005 BBa K784005]) and an engineered T7*(N4) RNA polymerase ([https://parts.igem.org/Part:BBa_K784044 K784044]). The riboswitch contains a RBS and it will be exposed and starts translating the T7*(N4) RNAP only after inserting of theophylline. The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7*(N4) RNAP are adjacent to one another without any restriction site between them. |
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Revision as of 10:37, 26 September 2012
theophylline riboswitch +T7*(N4) RNAP
This part contains an engineered theophylline riboswitch (RS) (BBa K784005) and an engineered T7*(N4) RNA polymerase (K784044). The riboswitch contains a RBS and it will be exposed and starts translating the T7*(N4) RNAP only after inserting of theophylline. The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7*(N4) RNAP are adjacent to one another without any restriction site between them.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 15
- 1000COMPATIBLE WITH RFC[1000]