Difference between revisions of "Part:BBa K909009"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K909009 short</partinfo> | <partinfo>BBa_K909009 short</partinfo> | ||
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+ | <!-- [[Image:UVR8.png|frameless|160px|right|thumb| UVR8 as a symmetric homodimer. Upon UV-B exposure the the dimer dissociates into two monomers. | ||
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UVR8 is responsible for UV-B detection in plants. In dark state UVR8 forms a dimer, which breaks into monomers upon exposure to UV-B light (280-315 nm). Later monomer induces transcriptional changes necessary for UV-B damage repairs and protection. | UVR8 is responsible for UV-B detection in plants. In dark state UVR8 forms a dimer, which breaks into monomers upon exposure to UV-B light (280-315 nm). Later monomer induces transcriptional changes necessary for UV-B damage repairs and protection. | ||
− | In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain), to use UVR8 as a UV-B sensing domain. | + | In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain (<partinfo>BBa_K909007</partinfo>)), to use UVR8 as a UV-B sensing domain (<partinfo>BBa_K909008</partinfo>). |
Revision as of 18:26, 26 September 2012
cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana
UVR8 is responsible for UV-B detection in plants. In dark state UVR8 forms a dimer, which breaks into monomers upon exposure to UV-B light (280-315 nm). Later monomer induces transcriptional changes necessary for UV-B damage repairs and protection.
In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain (BBa_K909007)), to use UVR8 as a UV-B sensing domain (BBa_K909008).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 560
Illegal PstI site found at 1214 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 76
Illegal PstI site found at 560
Illegal PstI site found at 1214 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 560
Illegal PstI site found at 1214 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 560
Illegal PstI site found at 1214 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 91