Difference between revisions of "Part:BBa K733012:Design"
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===Source=== | ===Source=== | ||
| − | We digest and ligate our xylR+PxylA+RBS+ydcE and pTms+RBS+ydcD. | + | We digest and ligate our [https://parts.igem.org/Part:BBa_K733011 xylR+PxylA+RBS+ydcE] and [https://parts.igem.org/Part:BBa_K733010 pTms+RBS+ydcD]. |
Backbone: pTms+RBS+ydcD in pSB1C3. Enzymes used: EcoRI and XbaI. | Backbone: pTms+RBS+ydcD in pSB1C3. Enzymes used: EcoRI and XbaI. | ||
Revision as of 17:58, 22 September 2012
xylR+PxylA+RBS+ydcE+Ptms+RBS+ydcD: Cell Growth Inhibition Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 847
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None.
Source
We digest and ligate our xylR+PxylA+RBS+ydcE and pTms+RBS+ydcD.
Backbone: pTms+RBS+ydcD in pSB1C3. Enzymes used: EcoRI and XbaI.
Insert: xylR+PxylA+RBS+ydcE. Enzyme used: EcoRI and SpeI. Purified by gel purification.