Difference between revisions of "Part:BBa K934012:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K934012=== | ===Applications of BBa_K934012=== | ||
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| + | [[Image:Plas-LuxI_result.png|thumb|center|500px|]] | ||
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| + | To characterize Plas-LuxI (BBa-K934012), we introduced Plas-LuxI (BBa-K934012) with Ptrc-LasR to E.coli as “3OC12HSL dependent 3OC6HSL producer cell”. In this E.coli, constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR ([https://parts.igem.org/Part:BBa_S03119 BBa-S03119]) and Plux-GFP ([https://parts.igem.org/Part:BBa_K395100 BBa-K395100]) to E.coli as a “Lux reporter cell”. | ||
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| + | In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI (BBa-K934012) synthesized 3OC6HSL. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 19:08, 25 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K934012
To characterize Plas-LuxI (BBa-K934012), we introduced Plas-LuxI (BBa-K934012) with Ptrc-LasR to E.coli as “3OC12HSL dependent 3OC6HSL producer cell”. In this E.coli, constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR (BBa-S03119) and Plux-GFP (BBa-K395100) to E.coli as a “Lux reporter cell”.
In the presence of 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, “Lux reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Lux reporter cell” is dually regulated by 3OC6HSL produced by “3OC12HSL dependent 3OC6HSL producer cell”, this result shows that Plas-LuxI (BBa-K934012) synthesized 3OC6HSL.
User Reviews
UNIQ6263e24f221ad0d7-partinfo-00000000-QINU UNIQ6263e24f221ad0d7-partinfo-00000001-QINU