Difference between revisions of "Part:BBa K784018"

 
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<partinfo>BBa_K784018 short</partinfo>
 
<partinfo>BBa_K784018 short</partinfo>
  
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Hila, update this.
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This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br>
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Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome. <br>
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Fragment 4 contains the phages tail proteins, which together with fragment 1 [[Part:BBa_K784015| (BBa_K784015)]], 2 [[Part:BBa_K784016| (BBa_K784016)]], and 3 [[Part:BBa_K784017| (Part:BBa_K784017)]] create the phage envelope. <br>
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<!-- Add more about the biology of this part here

Revision as of 16:42, 21 September 2012

Phage Lambda fragment 4 - tail fiber proteins

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome.
Fragment 4 contains the phages tail proteins, which together with fragment 1 (BBa_K784015), 2 (BBa_K784016), and 3 (Part:BBa_K784017) create the phage envelope.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal XbaI site found at 5029
    Illegal PstI site found at 354
    Illegal PstI site found at 802
    Illegal PstI site found at 2942
    Illegal PstI site found at 7449
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal PstI site found at 354
    Illegal PstI site found at 802
    Illegal PstI site found at 2942
    Illegal PstI site found at 7449
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal BglII site found at 2946
    Illegal BamHI site found at 2867
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal XbaI site found at 5029
    Illegal PstI site found at 354
    Illegal PstI site found at 802
    Illegal PstI site found at 2942
    Illegal PstI site found at 7449
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal XbaI site found at 5029
    Illegal PstI site found at 354
    Illegal PstI site found at 802
    Illegal PstI site found at 2942
    Illegal PstI site found at 7449
    Illegal NgoMIV site found at 561
    Illegal AgeI site found at 144
    Illegal AgeI site found at 215
    Illegal AgeI site found at 2599
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 7755
    Illegal SapI.rc site found at 5290