Difference between revisions of "Part:BBa K784009"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K784009 short</partinfo> | <partinfo>BBa_K784009 short</partinfo> | ||
− | This | + | This part has been created by deleting the aptamer and the additional 5' UTR regions of the [[Part:BBa_K784005|theophylline riboswitch]] using PCR. In addition, the deleted regions were replaced by a spacer region, which was added using the primers. The spacer region contains a PacI restriction site. As a result, only the RBS from the [[Part:BBa_K784005|theophylline riboswitch]] remains.<br> |
+ | Due to a mutation, this part differs from [[Part:BBa_K784007|BBa_K784007]]. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 14:09, 16 September 2012
Spacer2+RBS+mCherry
This part has been created by deleting the aptamer and the additional 5' UTR regions of the theophylline riboswitch using PCR. In addition, the deleted regions were replaced by a spacer region, which was added using the primers. The spacer region contains a PacI restriction site. As a result, only the RBS from the theophylline riboswitch remains.
Due to a mutation, this part differs from BBa_K784007.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]