Difference between revisions of "Part:BBa K559011:Design"
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Synthesized part by GenScript,all the illegal sites for RFC 10 (EcoRI, XbaI, SpeI, PstI) have been substituted by other codon producing the same amino acid in E. coli. | Synthesized part by GenScript,all the illegal sites for RFC 10 (EcoRI, XbaI, SpeI, PstI) have been substituted by other codon producing the same amino acid in E. coli. | ||
| − | + | This biobrick is connected to <partinfo>BBa_E0040</partinfo> through 3A assembly, with obeying RFC10 rules. | |
===Source=== | ===Source=== | ||
Latest revision as of 19:47, 2 November 2011
Pgad - Intracellular Chloride-Sensing Cassette
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Synthesized part by GenScript,all the illegal sites for RFC 10 (EcoRI, XbaI, SpeI, PstI) have been substituted by other codon producing the same amino acid in E. coli.
This biobrick is connected to BBa_E0040 through 3A assembly, with obeying RFC10 rules.
Source
The gene sequence was obtained from the cassette from bp 821 to 2071 of GenBank sequence AF005098, which includes PgadR, gadR, Pgad and the starting codon ATG.
References
[1]J.W. Sanders, G. Venema, and J. Kok, “A chloride-inducible gene expression cassette and its use in induced lysis of Lactococcus lactis,” Applied and environmental microbiology, vol. 63, Dec. 1997, p. 4877.
[2]J.W. Sanders, G. Venema, and J. Kok, “Identifcation of a sodium chloride-regulated promoter in Lactococcus lactis by single-copy chromosomal fusion with a reporter gene” Molecular Genetics & Genomics, vol. 257, Dec. 1988, p. 681-685.