Difference between revisions of "Part:BBa J176041:Design"
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Revision as of 16:03, 5 October 2011
Silver scar
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The six base pair scar allows protein modules to be assembled in frame. This scar retains the triplet pattern for proper translation of the mRNA. Two extra amino acids, Thr and Arg, are introduced into the protein fusion, but in our hands, this does not disrupt the function of our synthetic mammalian parts.
Source
This sequence is generated when BioBricks are assembled via assembly standard 23 (Silver standard).
References
Assembly standard 23 (Silver standard) https://parts.igem.org/Silver_standard