Difference between revisions of "Part:BBa R0079:Experience"

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<I>Tokyo-tech iGEM 2011</I>
 
<I>Tokyo-tech iGEM 2011</I>
 
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Judging from Northwestern iGEM 2011 team's data, in the presence of 3O-C12-HSL, the culture showed about 3-fold higher fluorescence intensity than that in the absence of 3O-C12-HSL.  
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Judging from Northwestern iGEM 2011 team's data, in the presence of 3O-C12-HSL, fluorescence intensity was about 3-fold higher than that in the absence of 3O-C12-HSL.  
  
  

Revision as of 03:39, 2 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_R0079

User Reviews

UNIQff1b18830dd7f1b9-partinfo-00000000-QINU UNIQff1b18830dd7f1b9-partinfo-00000001-QINU

No review score entered. NYMU-Taipei 2009

NYMU 2009-09-29.png We have characterised the strength of the promoters pCI, p22, pLux, pLas relative to pCI. More details are available at the [http://2009.igem.org/Team:NYMU-Taipei/Project/Promoter_Strength_Testing NYMU-Taipei iGEM09 wiki].
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No review score entered. Northwestern 2011

The Northwestern iGEM team used this part as a unit within our Pseudomonas Aeruginosa biosensor. When this LasR/PAI regulated promoter is induced at varying concentrations of PAI in the presence of excess LasR, we observed GFP fluorescence in accordance with the graph below.


LasR graph.jpg

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Tokyo-tech iGEM 2011

Judging from Northwestern iGEM 2011 team's data, in the presence of 3O-C12-HSL, fluorescence intensity was about 3-fold higher than that in the absence of 3O-C12-HSL.


We improved this part. LasI promoter(BBa_K649000) which we constructed was more successfully regulated by 3O-C12-HSL.


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