Part:BBa_R0053

Promoter (p22 cII regulated)

The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it.

This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)

Usage and Biology

p22 cII is a member of the lamboid cI protein family. Weak-medium promoter. [jb, 5/24/04] Note: not compatible with B0030 rbs due to likely transcript secondary structure This combination yielded very low gfp expression (see BBa_I7108). [jcb 8/3/05].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]